Key Features

      • Gene chip compatible with IVT (3’ expression arrays such as U133).
      • Gene chip compatible with all Exon and Gene ST Arrays.
      • Find genes with tissue specific expression.
      • Find genes with tissue specific alt. splicing.
      • Restrict to splicing that may change protein sequence.
      • Find outlier probes and samples.
      • Alternative splicing effect removed from gene level and fold change.
      • Auto generates a ‘methods document’ in MS Word.

Affymetrix GeneChip compatible

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Biotique XRAY

XRAY (Excel® Array Analysis) is an add-in for Microsoft® Excel® that finds significant gene expression level and alternative splicing differences between biological states

XRAY Overview

Familiar, User Friendly Environment
You already know how to use Excel so why learn another complicated gene expression application? The XRAY project set-up wizard gives you an easy interface to the most sophisticated analyses fully indexed by help and references.

All graphics and results are fully customizable and publication quality Excel graphs and tables. With XRAY’s easy to use interface you can be running your data in less than one hour.

Powerful, Fast Performance
Quickly run thousands of arrays on modest computers. Fixed memory implementation allows simultaneous analysis and normalization on computers with 500Mb ram. Excel 2002,2003, 2007 with Windows XP or Vista 32 or 64 bit. Mac running Windows supported.

Tiling Arrays
The XRAY wizard allows easy configuration of tiling analysis over multi-chip sets. MAT and TAS like algorithms are supported as well as Biotique’s implementation of an HMM to identify binding regions.

Intuitive Operation
The Analysis wizard makes project construction simple. Significant differential gene expression and alternative splicing events are represented in easy-to-understand result pages. The array quality report worksheet shows CEL File quality and normalization diagnostics to help you easily identify bad hybridization & artifact.

No Hassle Integration and Results Sharing
      • XRAY is unique in that you can share results with colleagues even if they don’t have an XRAY license.
      • Single button click of exon/gene results with expression values to UCSC tracks.
      • Automatic generation of MS Word document describintg methods and results.
      • IPA file generation for integration with Ingenuity™ Pathway Analysis.
      • Templates for R integration auto generated.
XRAY accepts binary CEL files, and integrates results with NetAffx and public databases. Excel offers easy integration with other office applications & ODBC databases. Users have full access to filtering, pivot tables, and all Excel functions.

Affordable Pricing
Academics: $1500 - Commercial: $3000

Screen Shots

Unique XRAY gene-page gives a single view of gene level, probe-set level, chip background, probe level, and biological annotation. The light background shows tissue specific exon usage between heart (red), skeletal muscle (dark blue), and other (light blue) tissues. The overlap of alt. splicing with the red triangles indicate that the alternative splicing may change protein sequence. Splicing patterns of known mRNAs agree with the expression analysis and are shown just below the triangles.

XRAY Customer Testimonials

" Gene expression analysis holds the key to navigating a wealth of information and I wanted to use expression data in my work. As a molecular biologist, I didn't know where to start in performing comprehensive expression analysis. XRAY offered the perfect solution with a step-by-step wizard that helped me set up my project and introduced me to the previously unfamiliar world of expression analysis."

- Takatoku Oida, Harvard University